WebIn this paper, we provide a general protocol for labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder-ethanedithiol (FlAsH-EDT₂).... Webdyes, such as fluorescein arsenical hairpin binder (FlAsH) and resorufin arsenical hairpin binder (ReAsH) [16, 17]. FlAsH or ReAsH are prepared as ethane dithiol (EDT) …
Protein labeling with FlAsH and ReAsH - PubMed
Web@journal { , title = {Fluorescent labeling of tetracysteine-tagged proteins in intact cells}, address = {}, booktitle = {Nature Protocols}, chapter = {}, edition ... WebJun 28, 2024 · The fluorescein arsenical hairpin binder (FlAsH) fluorescein tag contains the amino acid sequence of CCPGCC, within which the tetracysteine motif is labeled with FlAsH-ETD2 fluorescein (Fig. 1b ). Fig. 1 pOPHBL and pOPHFL bacterial expression vectors. ( a) A map of the pOPHBL plasmid. DNA sequences and their amino acid … incompetent\\u0027s ys
Fluorescent labeling of tetracysteine-tagged proteins in intact …
FlAsH-EDT2 is an organoarsenic compound with molecular formula C24H18As2O5S4. Its structure is based around a fluorescein core with two 1,3,2-dithiarsolane substituents. It is used in bioanalytical research as a fluorescent label for visualising proteins in living cells. FlAsH-EDT2 is an abbreviation for … See more FlAsH-EDT2 can be prepared in three steps from fluorescein (see figure). See more Many studies show that trivalent arsenic compounds bind to pairs of cysteine residues. This binding is responsible for the toxicity of many arsenic compounds. Binding is reversed … See more FlAsH-EDT2 enables less toxic and more specific fluorescent labeling that is membrane permeable. The modification of the fluorescein moiety also allows multicolor analysis. … See more FlAsH becomes fluorescent upon the binding of tetracysteine motif. It is excited at 508 nm and emits 528 nm, a green-yellow, of free fluorescein. The quantum yield is 0.49 for 250 nM FlAsH is bound to a model tetracysteine-containing peptide in a phosphate … See more WebOct 12, 2024 · The arsenical hairpin derivative of fluorescein, FlAsH, offers a highly specific labelling strategy of proteins via a small tetracysteine motif (FCM) which can be … WebThe receptors were C-terminally labeled with cyan fluorescent protein and with fluorescein arsenical hairpin binder at different sites in the third intracellular loop: N-terminally close to transmembrane domain V (I3-N), in the middle of the loop (I3-M), or C-terminally close to transmembrane domain VI (I3-C). incompetent\\u0027s yp