Phospho nfat flow antibody
WebWhen selecting a phospho-specific antibody for your experiments, it is crucial to ensure that the antibody specifically detects the protein of interest only when it is phosphorylated at the indicated site. It is also essential to check that the antibody detects a band at the correct molecular weight for your phosphorylated target protein. WebPhospho-specific antibodies allow for the phosphorylation state of individual protein residues to be determined by standard, immunoblotting techniques.26 Several phospho-specific antibodies have been developed for Raf-1, including antibodies that recognize phospho-Ser-338 and phospho-Tyr-340/341.
Phospho nfat flow antibody
Did you know?
WebThe NFAT2 (phospho-Ser233) (phospho-Ser235) antibody from Biorbyt is a Rabbit Polyclonal antibody to NFATC1. This antibody recognizes Human, and Rat antigen. The NFAT2 (phospho-Ser233) (phospho-Ser235) antibody has been validated for the following applications: EIA, Immunoassay, ELISA, Immunocytochemistry, Immunofluorescence, … WebAnalysis was performed after exposure to drugs (CEP-701 and SU11657), in vitro and in vivo. In response to increasing drug concentrations, there was a linear reduction in P-FLT3. Intracellular flow cytometry analysis correlated with Western blot and XTT assays; flow cytometry data also correlated with FLT3 mutational status.
WebRabbit polyclonal NFAT1 (phospho S54) antibody. Validated in WB and tested in Mouse. Cited in 3 publication(s). Immunogen corresponding to synthetic peptide. WebThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application ICFC - Quality tested. Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis.
WebDec 26, 2024 · Phosphate is one of the most important molecular elements to normal cellular functions within the body. It acts as an integral component of nucleic acids and is used to replicate DNA and RNA. It is an … WebMay 14, 2024 · For both NFAT-mediated GFP ... Cell pellets were resuspended in the residual media by pulsed vortexing. When required, primary antibodies at ~10 µg/ml, diluted in flow wash buffer (100 µl ... The resuspended pellet was then incubated with the phospho-ERK1/2 (Thr 202 /Tyr 204) antibody directly conjugated with Alexa Fluor 488 for 45 min at ...
WebNFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation …
WebThe NFAT (nuclear factor of activated T cells) family of proteins consists of NFAT1 (NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx). All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective ... rawle murdy associatesWebThis antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I). rawle murdy associates incWebJun 27, 2011 · Nuclear factor of activated T cells (NFAT) proteins are Ca 2+ -regulated transcription factors that control gene expression in many cell types. NFAT proteins are … rawlence road salisburyWebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold rawle mitchellWebAnti-phospho-NFAT1 (pSer54) antibody produced in rabbit (affinity isolated antibody, buffered aqueous solution); Suitable for western blot; Rabbit polyclonal anti-phospho-NFAT1 (pSer54) antibody is used to tag phospho-NFAT1 (pSer54) for detection and quantitation by immunocytochemical and USEN ApplicationsProductsServicesSupport N8536 All Photos(1) rawlemon prixWebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. rawle meaningWebThis antibody conjugate is suitable for intracellular staining of human cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used. Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. rawle law firm