The oligonucleotide probe database
WebJan 1, 2003 · In 1996, Alm and coworkers compiled the Oligonucleotide Probe Database (OPD) that listed 96 PCR primers and probes mainly targeting small-subunit (SSU) and … WebOct 24, 2024 · Oligonucleotide probes. Oligonucleotide probes are short stretches of single-stranded DNA or RNA used to detect the presence of complementary nucleic acid …
The oligonucleotide probe database
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WebAug 24, 2015 · PrimeTime Predesigned qPCR Sequence Database Select primers/probes for qPCR (human, mouse, rat transcriptome). ... Minimum, optimum, and maximum melting temperature for a primer oligo (range 45–75°C) Primer criteria—primer GC (%) Minimum, optimum, and maximum percentage of G and C bases in the primer (range: 20‑80%) WebJul 5, 2024 · While tools exist to efficiently generate genome-scale sets of oligo probes 33,34, to the best of our knowledge no comprehensive database exists to connect the coordinates of the discovered probes ...
WebFinally, multiple Label Probes hybridize to the Amplifiers to form the “leaves” of the tree. A fully assembled signal amplification tree contains 400 Label Probe binding sites. If all target-specific oligonucleotides in a 20-oligonucleotide-pair Probe Set bind to the target RNA transcript, an 8,000-fold amplification can be achieved. WebOligonucleotide Probes for Fluorescence In Situ Hybridization Philip Hugenholtz, Gene W. Tyson, and Linda L. Blackall 1. Introduction Fluorescence in situ hybridization (FISH) of whole cells using 16S rRNA-targeted oligonucleotide probes is a powerful technique with which to evaluate
WebAnother strategy of probe selection is searching for already published probes. Loy and coworkers established an rRNA-targeted oligonucleotide probe database named 'probeBase' with currently more than 1300 entries, … WebApr 26, 2014 · Here, we present PhylOPDb (‘phylogenetic oligonucleotide probe database’), a comprehensive phylogenetic OPD targeting 16S rRNA gene sequences. We used two high-throughput probe design software, PhylGrid and KASpOD, to select both regular and explorative 16S rRNA gene-targeted oligonucleotide probes. PhylOPDb is composed of 74 …
WebOct 1, 2008 · OligoArrayDb is a comprehensive database containing pangenomic oligonucleotide microarray probe sets designed for most of the sequenced genomes that are not covered by commercial catalog arrays.
WebJan 1, 2024 · Here, we present Oligonucleotide Capture Probes for Pathogen Identification Database (OCaPPI-Db), an online capture probe database containing a set of 1,685 … texas new chief schoolWebNov 19, 2024 · 2. COMBO-FISH: principle and applications. In contrast to standard FISH where probes are usually cut and amplified by molecular biology techniques, COMBinatorial Oligonucleotide FISH (COMBO-FISH) follows a completely different strategy for probe design [19, 20, 26, 27].If a genome of a species is sequenced and the sequence is … texas new cdl lawWeb· Each probe set must contain at least three oligonucleotide probes and probes in a set are ordered according to their genomic location. 2.2. CDF files for Reference sequence, Entrez Gene and Exon, ENSEMBL Gene, Transcript and Exon and VEGA Gene, Transcript and Exon · A probe must hit only one genomic location texas new channelWebProbe contains sequences of oligonucleotide probes and results from a wide variety of applications and technologies, including gene silencing experiments (RNAi), gene expression assays, variation analysis, genome mapping, nucleic acid detection, and genotyping studies. ... The Probe database is now searchable as a part of the Entrez database ... texas new chief development programWebThe oligonucleotide probe database. Applied and Environmental Microbiology. 1996;62(10):3557-3559. doi: 10.1128/aem.62.10.3557-3559.1996. Powered by Pure, … texas new cdWebJan 1, 2006 · Easy access to the primer and probe data is possible by using multiple search criteria. Assay reports contain gene information, assay details (such as oligonucleotide sequences, detection chemistry and reaction conditions), publication information, users' experimental evaluation feedback and submitter's contact details. texas new child support lawsWebJan 1, 2024 · Here, we present Oligonucleotide Capture Probes for Pathogen Identification Database (OCaPPI-Db), an online capture probe database containing a set of 1,685 oligonucleotide probes allowing for the detection and identification of 30 biothreat agents up to the species level. texas new china king buffet