The solubility of hen egg-white lysozyme
WebFeb 16, 1999 · For example, the solubility of the well studied and representative hydrophilic protein hen egg-white lysozyme (pI ≈11) ( 3 ), lyophilized from pH 6, exceeds 10 mg/ml in more than a dozen nonaqueous solvents without any extra additives ( 1 ). WebNov 8, 2010 · The results demonstrate that lysozyme solubility increases with the addition concentration of [C4mim]BF4 and [C4mimCl] and is nearly invariable with the increase of the [C4mim]Br concentration. ... Ionic liquids as selectors for controlling the crystallization nucleation of hen egg white lysozyme. Biotechnology and Bioprocess Engineering 2012 ...
The solubility of hen egg-white lysozyme
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WebFeb 16, 1999 · Hen egg-white lysozyme dissolved in glycerol containing 1% water was studied by using CD and amide proton exchange monitored by two-dimensional 1 H NMR. …
WebIn the present study, based on spectroscopic and high sensitivity calorimetric data we have explored individual as well as the combined effect of two osmolytes; trehalose and glycine on conformation and stability of hen egg white lysozyme. Glycine induced greater thermal stability to lysozyme compared to that by trehalose. WebHen egg white is a commercially available mixture of proteins that can yield purified lysozyme using a combination of conventional processes.
WebFeatures of Lysozyme lyophilized powder: • Bacterial cell wall lytic enzyme that improves inclusion body protein purification • Compatible with Thermo Scientific Pierce Cell Lysis Reagents • White, lyophilized powder • Approx. … WebJan 1, 1998 · The solubility of chicken egg white lysozyme, crystallized in the tetragonal form was measured in sodium chloride solutions from 1.6 to 30.7 C, using a miniature column solubility apparatus. Sodium chloride solution concentrations ranged from 1 to 7% (w/v). The solutions were buffered with 0.1 M sodium acetate buffer with the solubility …
WebMay 1, 2008 · To observe the ionized status of the amino acid residues in proteins at different pH (protein pH titration in the crystalline state) by neutron diffraction, hen egg-white lysozyme was...
WebNov 1, 1997 · When hen egg-white lysozyme was produced in Escherichia coli, it possessed an extra methionine residue at the N-terminus (Met(-1)-lysozyme). The Met(-1)-lysozyme showed a decreased refolding yield and solubility compared with the native hen egg-white lysozyme, as the methionine is a hydrophobic amino acid. crescent ridge hoursWebThe solubility of the tetragonal form of hen egg white lysozyme from pH 4.0 to 5.4 Elizabeth L. Cacioppo, M. Pusey Chemistry 1991 201 Variation of lysozyme solubility as a function … bu commandWebNov 1, 1991 · Hen egg white lysozyme solubilities in the presence of the tetragonal crystal form have been determined. Conditions investigated cover the pH range 4.0 to 5.4, varying … bucom st650exWebApr 15, 2024 · The characteristics of the translational mobility obtained by NMR with PFG demonstrated the peculiarities of the interaction of HAs with bovine serum albumin (BSA) … crescent road consulting roomsWebThe problem of the relative efficiency of various ions in the lysozyme crystallization has been considered for many years [1,2].Hofmeister in reference [] showed that the solubility of lysozyme mainly depends on anions and only slightly on cations.In reference [], it was also found that according to the order of effectiveness of salts with different anions (pTs −, … bucomix medicationWebNov 8, 2010 · The results demonstrate that lysozyme solubility increases with the addition concentration of [C4mim]BF4 and [C4mimCl] and is nearly invariable with the increase of … crescent road master planWebThe mechanism behind its action is still not fully understood. Using hen egg white lysozyme as a model protein, we present data that clearly demonstrate the suppression of the aggregation of denatured protein by L-Arg. B … L-Arginine increases the solubility of unfolded species of hen egg white lysozyme Protein Sci. bu computer science master